Procalcitonin and C-reactive protein to rule out early bacterial coinfection in COVID-19 critically ill patients.

PURPOSE: Although the prevalence of community-acquired respiratory bacterial coinfection upon hospital admission in patients with coronavirus disease 2019 (COVID-19) has been reported to be < 5%, almost three-quarters of patients received antibiotics. We aim to investigate whether procalcitonin (PCT) or C-reactive protein (CRP) upon admission could be helpful biomarkers to identify bacterial coinfection among patients with COVID-19 pneumonia. METHODS: We carried out a multicentre, observational cohort study including consecutive COVID-19 patients admitted to 55 Spanish intensive care units (ICUs). The primary outcome was to explore whether PCT or CRP serum levels upon hospital admission could predict bacterial coinfection among patients with COVID-19 pneumonia. The secondary outcome was the evaluation of their association with mortality. We also conducted subgroups analyses in higher risk profile populations. RESULTS: Between 5 February 2020 and 21 December 2021, 4076 patients were included, 133 (3%) of whom presented bacterial coinfection. PCT and CRP had low area under curve (AUC) scores at the receiver operating characteristic (ROC) curve analysis [0.57 (95% confidence interval (CI) 0.51-0.61) and 0.6 (95% CI, 0.55-0.64), respectively], but high negative predictive values (NPV) [97.5% (95% CI 96.5-98.5) and 98.2% (95% CI 97.5-98.9) for PCT and CRP, respectively]. CRP alone was associated with bacterial coinfection (OR 2, 95% CI 1.25-3.19; p = 0.004). The overall 15, 30 and 90 days mortality had a higher trend in the bacterial coinfection group, but without significant difference. PCT ≥ 0.12 ng/mL was associated with higher 90 days mortality. CONCLUSION: Our study suggests that measurements of PCT and CRP, alone and at a single time point, are not useful for ruling in or out bacterial coinfection in viral pneumonia by COVID-19.

Anomie, irritation, and happiness in the Chilean society post-social outbreak.

On 18 October 2019, the Chilean people witnessed an unprecedented social outbreak across most of their country. We argue that a state of anomie is a factor associated with the weakening of states, and an anomic state might negatively influence people's well-being through an increased feeling of irritation. Convenience recruitment via social networks allowed us to form a sample of 194 Chilean participants from the center-south region of the country (M = 36.53 years old, SD = 17.48; 56.7% women). All participants completed testing instruments to measure anomie, irritation, happiness, and political beliefs. Descriptive scores suggest situating Chile in the quadrant of high anomie. Two mediation analyses were conducted. The main results showed a negative indirect effect of the breakdown of the social fabric and leadership on happiness through irritation, although the findings for the former dimension were more robust. Additionally, the breakdown of the social fabric was positively related to the belief that left and right-wing democratic governments are helpless when it comes to fighting delinquency. The breakdown of leadership, on the other hand, was negatively related to political interest. The results should be interpreted with caution due to the limitations of the sample type and the construction validity of some instruments.

The role of vitamin D on redox regulation and cellular senescence.

Vitamin D is considered an essential micronutrient for human health that is metabolized into a multifunctional secosteroid hormone. We can synthesize it in the skin through ultraviolet B (UVB) rays or acquire it from the diet. Its deficiency is a major global health problem that affects all ages and ethnic groups. Furthermore, dysregulation of vitamin D homeostasis has been associated with premature aging, driven by various cellular processes, including oxidative stress and cellular senescence. Various studies have shown that vitamin D can attenuate oxidative stress and delay cellular senescence, mainly by inducing the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and Klotho and improving mitochondrial homeostasis, proposing this vitamin as an excellent candidate for delaying aging. However, the mechanisms around these processes are not yet fully explored. Therefore, in this review, the effects of vitamin D on redox regulation and cellular senescence are discussed to propose new lines of research and clinical applications of vitamin D in the context of age-related diseases.

Dexamethasone as risk-factor for ICU-acquired respiratory tract infections in severe COVID-19.

PURPOSE: Dexamethasone is the only drug that has consistently reduced mortality in patients with COVID-19, especially in patients needing oxygen or invasive mechanical ventilation. However, there is a growing concern about the relation of dexamethasone with the unprecedented rates of ICU-acquired respiratory tract infections (ICU-RTI) observed in patients with severe COVID-19. METHODS: This was a multicenter, prospective cohort study; conducted in ten countries in Latin America and Europe. We included patients older than 18 with confirmed SARS-CoV-2 requiring ICU admission. A multivariate logistic regression and propensity score matching (PSM) analysis was conducted to determine the relation between dexamethasone treatment and ICU-RTI. RESULTS: A total of 3777 patients were included. 2065 (54.7%) were treated with dexamethasone within the first 24 h of admission. After performing the PSM, patients treated with dexamethasone showed significantly higher proportions of VAP (282/1652 [17.1%] Vs. 218/1652 [13.2%], p = 0.014). Also, dexamethasone treatment was identified as an adjusted risk factor of ICU-RTI in the multivariate logistic regression model (OR 1.64; 95%CI: 1.37-1.97; p < 0.001). CONCLUSION: Patients treated with dexamethasone for severe COVID-19 had a higher risk of developing ICU-acquired respiratory tract infections after adjusting for days of invasive mechanical ventilation and ICU length of stay, suggesting a cautious use of this treatment.

Laparoscopic-guided distal loco-regional anesthetic infiltration technique in TAPP inguinal hernia repair: a double-blind randomized clinical trial.

BACKGROUND: Ultrasound-guided transversus abdominis plane block (US-TAP) is an important component of multimodal analgesia in laparoscopic inguinal hernia repair, although it has certain limitations. To overcome them, surgeons have developed several techniques to perform local anesthetic infiltration under laparoscopic guidance, but no trials evaluating these in transabdominal preperitoneal (TAPP) hernia repair were conducted till the date. The aim of this study was to compare the efficacy of a novel laparoscopic-guided local anesthetic infiltration technique (LDAI) with US-TAP in postoperative pain control and analgesic consumption for patients undergoing elective TAPP hernia repair. METHODS: This was a double-blind randomized controlled trial conducted at a single tertiary academic center between 2019 and 2020 on adult patients undergoing elective laparoscopic TAPP inguinal hernia repair. Postoperative pain and analgesic consumption were compared for LDAI vs. US-TAP up to 30 postoperative days. RESULTS: 62 patients were included (31 LDAI, 31 US-TAP). Female gender was significantly higher in the LDAI group (8, 25.81%; US-TAP 0; p = 0.005). Mean anesthetic time (US-TAP group: 142.2 min, SD = 17.7; LDAI group: 127.1 min, SD = 15.5; p < 0.001) and mean operative time (US-TAP group: 117.2 min, SD = 15.9; LDAI group: 103.8 min, SD = 15.2; p < 0.001) were significantly shorter in the LDAI group. Pain scores assessed at the first-hour postoperative, at the moment of discharge, and at 8, 24, and 48 postoperative hours showed no significant differences between both groups. No significant difference was found regarding postoperative analgesic rescue administration in the recovery room and analgesic consumption after discharge between groups. CONCLUSION: LDAI is a safe and effective local anesthetic technique in elective TAPP hernia repair. Pain control is similar to US-TAP block, with shorter anesthesthetic and surgical time and better health resources allocation.

Quality of Life and Caregiver Burden of Alzheimer's Disease Among Community Dwelling Patients in Europe: Variation by Disease Severity and Progression.

BACKGROUND: Alzheimer's disease (AD) is a significant burden on patients and caregivers. How this burden increases as disease progresses has not been well researched. OBJECTIVE: To assess the association of caregiver burden and quality of life with Alzheimer's disease severity and disease progression in community-dwelling patients in Germany, Spain, and the UK. METHODS: This was a prospective observational longitudinal study of mild-to-moderate AD patients (assessed by Mini-Mental State Examination, MMSE), and their caregivers. The humanistic burden was assessed using these instruments: [Rapid Assessment of Physical Activity (RAPA), EuroQoL-5-Dimension Level (EQ-5D-5L)] and caregiver-reported [Dependence Scale (DS), EQ-5D-5L, Zarit Burden Interview (ZBI)]. Caregiver-reported healthcare resource use was assessed using the Resource Use in Dementia (RUD) and ad-hoc questions. RESULTS: Of 616 patients recruited, 338 and 99 were followed-up at 12 and 18 months, respectively. The caregiver-reported EQ-5D-5L scores of patients' health-related quality of life (HRQoL) showed a negative trend over time (baseline: 0.76; 18 months: 0.67) while patient-reported HRQoL remained at 0.85. DS scores tended to worsen. Disease progression was an independent predictor of HRQoL and increased dependence.Mean ZBI score increased over time reflecting an increase in caregiver burden; MMSE being an independent predictor for caregiver burden. Patient resource utilization and caregiver time for caregiving tended to increase over time. CONCLUSION: We found significant association between disease progression and caregiver and patient burden. Independently, both disease-specific outcomes and disease burden measures increased over time, but as disease progresses, we also found incremental burden associated with it.

Yes! Maximizers Maximize Almost Everything: The Decision-Making Style Is Consistent in Different Decision Domains.

The decision-making literature distinguishes one maximizing style from another satisficing decision-making style, but it is unknown whether these styles remain stable or are variable depending on the occasion. One way to approach it is to verify eventual generalization of these styles in behavior of people in different decision domains. Some incipient results with University students from the United States and Austria suggest that these styles would remain in three different domains. However, it is unknown if this is the case in adults, other cultures, or vital areas of great relevance, such as health and personal finances. The objective here is to identify if Chilean Latin American participants of different sex and age maintain their decision-making style in five different decision domains. The sample was 343 volunteers, 52.6% men, from two regions of central-southern Chile (Maule and Ñuble), aged between 20 and 90 years (M = 45.47; SD = 16.05), who answered the Maximization Tendency Scale, and 45 items corresponding to five different decision domains: health, life decision, finances, services and experiences, and consumer's good. An apparent coherence of decision-making style-maximizing and satisficing-was obtained in the five domains. The health domain stands out for being the one in which it is maximized and with greater internal homogeneity.

Extracellular vesicle-associated miRNAs are an adaptive response to gestational diabetes mellitus.

BACKGROUND: Gestational diabetes mellitus (GDM) is a serious public health issue affecting 9-15% of all pregnancies worldwide. Recently, it has been suggested that extracellular vesicles (EVs) play a role throughout gestation, including mediating a placental response to hyperglycaemia. Here, we investigated the EV-associated miRNA profile across gestation in GDM, assessed their utility in developing accurate, multivariate classification models, and determined the signaling pathways in skeletal muscle proteome associated with the changes in the EV miRNA profile. METHODS: Discovery: A retrospective, case-control study design was used to identify EV-associated miRNAs that vary across pregnancy and clinical status (i.e. GDM or Normal Glucose Tolerance, NGT). EVs were isolated from maternal plasma obtained at early, mid and late gestation (n = 29) and small RNA sequencing was performed. Validation: A longitudinal study design was used to quantify expression of selected miRNAs. EV miRNAs were quantified by real-time PCR (cases = 8, control = 14, samples at three times during pregnancy) and their individual and combined classification efficiencies were evaluated. Quantitative, data-independent acquisition mass spectrometry was use to establish the protein profile in skeletal muscle biopsies from normal and GDM. RESULTS: A total of 2822 miRNAs were analyzed using a small RNA library, and a total of 563 miRNAs that significantly changed (p < 0.05) across gestation and 101 miRNAs were significantly changed between NGT and GDM. Analysis of the miRNA changes in NGT and GDM separately identified a total of 256 (NGT-group), and 302 (GDM-group) miRNAs that change across gestation. A multivariate classification model was developed, based on the quantitative expression of EV-associated miRNAs, and the accuracy to correctly assign samples was > 90%. We identified a set of proteins in skeletal muscle biopsies from women with GDM associated with JAK-STAT signaling which could be targeted by the miRNA-92a-3p within circulating EVs. Interestingly, overexpression of miRNA-92a-3p in primary skeletal muscle cells increase insulin-stimulated glucose uptake. CONCLUSIONS: During early pregnancy, differently-expressed, EV-associated miRNAs may be of clinical utility in identifying presymptomatic women who will subsequently develop GDM later in gestation. We suggest that miRNA-92a-3p within EVs might be a protected mechanism to increase skeletal muscle insulin sensitivity in GDM.

Sleep problems are related to commuting accidents rather than to workplace accidents.

BACKGROUND: This study aimed to verify the relationships between sleep problems and both commuting and workplace accidents in workers of both sexes. METHODS: The study was carried out with a sample of workers (n = 2993; 50.2% female) from the Chilean Quality of Life Survey (ENCAVI) 2015-2016, while the rates of both workplace and commuting accidents were extracted from the statistics of the Superintendence of Social Security (SUSESO 2015; 180,036 and 52,629 lost-time accidents, respectively). RESULTS: Chilean workers sleep less than the rest of the people in the country (MW = 7.14 vs. MO = 7.33; t (6789) = - 5.19; p < .001), while the Chilean people as a whole sleep less compared to those of other countries (7.24 h per day). Likewise, it was found that sleep problems are more strongly related to commuting than to workplace accidents. In this vein, sleep quantity can explain 24% of the variance in commuting accidents' rates (Stepwise Method; R2 = .30, F (1.14) = 5.49, p < .05; ß = -.55, p < .05), by using aggregated data with all types of commuting roles (driver of a vehicle, a passenger of public or private transport, or as a pedestrian). CONCLUSIONS: Our findings show that sleep quantity has a more robust relationship with commuting than workplace accidents, a neglected issue so far. Future prevention programs should emphasize sleep hygiene and focus on commuting to and from work.

Strategies for follow up after hernia surgery during COVID 19 Pandemia.

The end of 2019 was marked by the emergence of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Our problem is centered in the post operative follow up of those patients who underwent an elective procedure immediately before the isolation and those who require an emergency surgery. We outline the measures we have taken to reduce the possibility of spread of the virus.

New approaches to maximization: evidence of correlations with malaise and well-being in the chilean adult population / Nuevos enfoques de maximización: evidencia de correlaciones con malestar y bienestar en población chilena adulta

Abstract There is a lack of theoretical consensus about the decision-making process and particularly regarding the maximization construct. Recently, two conceptual approaches to maximization and their respective instruments were proposed. The first defines maximization as consisting of two dimensions, goal and strategy. The second differentiates between the two types of maximization according to the way an individual makes decisions, one is resolute and the other is fearful. Regarding the first approach, empirical goal and strategy relationships with emotional consequences on well-being and discomfort are unknown while deciding, while it is doubt whether the distinction proposed in the second approach also applies to the adult population and in contexts different from the European one. Empirical associations are evaluated here for each approach regarding indicators of malaise and well-being through a set of hypotheses. A sample of 624 Chilean adults of both sexes (20 to 70 years old) answered eight instruments on maximization, its components and types, and well-being and malaise. The results for both approaches show greater associations with rates of malaise than well-being. The results for the first approach show a more intense association with indices of malaise and well-being for strategy than goal. In the second approach, these associations are stronger and greater for fearful maximization than for resolute. The results contribute by increasing the understanding of the maximization construct by simultaneously showing that the increment in the strategy search for alternatives and the fearful style of maximization are directly associated with difficulty in deciding and regret.

Resumen Existe carencia de consenso teórico acerca del proceso de tomar decisiones y particularmente respecto del constructo maximización. Recientemente, se propuso dos enfoques conceptuales sobre maximización y sus respectivos instrumentos de evaluación. El primero considera que el concepto de maximización está constituido por dos dimensiones, meta y estrategia. El segundo diferencia entre dos tipos de maximización según el modo de decidir de un individuo, uno resuelto y otro temeroso. Respecto del primer enfoque se desconocen eventuales relaciones empíricas de meta y estrategia con consecuencias emocionales sobre el bienestar y malestar mientras se decide, mientras existe la duda si la distinción propuesta en el segundo enfoque se aplica también a población de adultos y en contextos diferentes al europeo. En el presente trabajo son evaluadas asociaciones empíricas para cada enfoque respecto de indicadores de malestar y bienestar mediante un conjunto de hipótesis. Una muestra de 624 adultos chilenos de ambos sexos (20 a 70 años) respondió ocho instrumentos sobre maximización, sus componentes y sus tipos, y su bienestar y malestar. Los resultados para ambos enfoques muestran asociaciones mayores con índices de malestar que de bienestar. Los resultados para el primer enfoque muestran asociación con índices de malestar y bienestar más intensos para la dimensión de estrategia que la de meta. En el segundo enfoque estas asociaciones son fuertes y mayores para el tipo de maximización temerosa que para resuelta. Los resultados contribuyen a incrementar la comprensión del constructo de maximización, al mostrar simultáneamente que el aumento en la estrategia de búsqueda de alternativas y el estilo temeroso de maximización, se asocian directamente con dificultad para decidir y con experimentar pesar.

Membership, Neighborhood Social Identification, Well-Being, and Health for the Elderly in Chile.

The world's elderly population is growing, and in Chile they represent 16.2% of the total population. In Chile, old age is marked by retirement, with a dramatic decrease in income that brings precariousness. Older adults are economically, socially, and psychologically vulnerable populations. This condition increases their likelihood of disengaging from their usual social environment, facilitating their isolation, sadness, and discomfort. From the perspective of social identity, well-being (WB) can be explained by two principles: social groups' importance for health and people's psychological identification with those groups. This study analyzes the relationships between belonging to the neighborhood and extra-neighborhood groups and neighborhood social identification with WB. Urban or rural location and gender are measured, and the sample is 1,475 older Chilean adults of both sexes. The results show that the majority are not members of social groups (52%), and the remaining 48% are members of one or two groups or organizations (42.65%). Only 4.47% belong to three groups or organizations. Those who belong to groups obtain higher scores, emotional-mental WB, and positive emotions than older adults who do not belong to any organization. Urban and rural older adults have the same level of WB. Membership in close social organizations (neighborhood councils) or distant ones (clubs for the elderly and religious groups) causes different WB associations. Membership in neighborhood councils reduces gender differences in self-assessment of health. This result supports the idea that participation in heterogeneous groups with a shared sense of belonging to the neighborhood is associated with higher WB and lower perceived loneliness. Social identification with the neighborhood, rather than belonging to the group, had the most widespread impact on WB and health indicators. The variable social identification with the neighborhood was consistently associated with indicators of hedonic WB.

Hacia la validación del SUSESO ISTAS 21 versión breve en trabajadores de hospitales públicos / Towards the validation of the SUSESO ISTAS 21 short version in workers of public hospitals

Resumen La mitad de la población mundial son trabajadores, por lo que invertir en políticas de salud hacia ellos es muy relevante. La Organización Mundial de la Salud (oms) insta a los estados miembros a generar planes de salud ocupacional evaluables para promover el bienestar de los trabajadores, para lo cual se requiere contar con instrumentos adecuados. Se estudian aquí propiedades psicométricas del instrumento para evaluar riesgo psicosocial SUSESO-ISTAS 21 versión breve (20 ítems), aplicado a 341 trabajadores hospitalarios (región del Maule, Chile), conjuntamente con la DASS21 (Depresión, Ansiedad y Estrés). La confiabilidad general es adecuada, reafirmando informes previos. Respecto de validez, cuatro de las cinco dimensiones de la versión breve no alcanzan valores psicométricos adecuados, no confirmándose la estructura factorial teorizada. Se obtiene apoyo a la validez convergente con DASS21. Se discute y propone acerca de lo psicométrico y sus usos eventuales.

Abstract Workers represent half of the world's population, so investing in public health policies aimed at them has enormous importance. The World Health Organization (who) urges member states to generate assessable occupational health plans that promote their wellbeing. The study aims to evaluate the psychometric properties of ISTAS-SUSESO 21 short version (20 items), which is an instrument for assessing psychosocial risk developed for Chile. We performed reliability analysis, confirmatory factor analysis using structural equation model and convergent validity analysis with the DASS21 Scale (Depression, Anxiety and Stress) using a sample of 341 hospital employees in the region of Maule (Chile). Overall reliability is adequate, reaffirming previous reports. Regarding validity, four of five dimensions of the short version does not reach adequate psychometric values, and theorized factorial structure is not confirmed. We found support for convergent validity with DASS 21, and discussed the psychometric and its possible uses.

Human placental exosomes in gestational diabetes mellitus carry a specific set of miRNAs associated with skeletal muscle insulin sensitivity.

There is increasing evidence that miRNAs, which are enriched in nanovesicles called exosomes, are important regulators of gene expression. When compared with normal pregnancies, pregnancies with gestational diabetes mellitus (GDM) are associated with skeletal muscle insulin resistance as well as increased levels of circulating placental exosomes. Here we investigated whether placental exosomes in GDM carry a specific set of miRNAs associated with skeletal muscle insulin sensitivity. Exosomes were isolated from chorionic villous (CV) explants from both women with Normal Glucose Tolerant (NGT) and GDM pregnancies. Using miRNA sequencing, we identified a specific set of miRNAs selectively enriched with exosomes and compared with their cells of origin indicating a specific packaging of miRNAs into exosomes. Gene target and ontology analysis of miRNA differentially expressed in exosomes secreted in GDM compared with NGT are associated with pathways regulating cell migration and carbohydrate metabolism. We determined the expression of a selected set of miRNAs in placenta, plasma, and skeletal muscle biopsies from NGT and GDM. Interestingly, the expression of these miRNAs varied in a consistent pattern in the placenta, in circulating exosomes, and in skeletal muscle in GDM. Placental exosomes from GDM pregnancies decreased insulin-stimulated migration and glucose uptake in primary skeletal muscle cells obtained from patients with normal insulin sensitivity. Interestingly, placental exosomes from NGT increase migration and glucose uptake in response to insulin in skeletal muscle from diabetic subjects. These findings suggest that placental exosomes might have a role in the changes on insulin sensitivity in normal and GDM pregnancies.

Radial Acceleration Relation of ΛCDM Satellite Galaxies.

The radial acceleration measured in bright galaxies tightly correlates with that generated by the observed distribution of baryons, a phenomenon known as the radial acceleration relation (RAR). Dwarf spheroidal satellite galaxies have been recently found to depart from the extrapolation of the RAR measured for more massive objects but with a substantially larger scatter. If confirmed by new data, this result provides a powerful test of the theory of gravity at low accelerations that requires robust theoretical predictions. By using high-resolution hydrodynamical simulations, we show that, within the standard model of cosmology (ΛCDM), satellite galaxies are expected to follow the same RAR as brighter systems but with a much larger scatter which does not correlate with the physical properties of the galaxies. In the simulations, the RAR evolves mildly with redshift. Moreover, the acceleration due to the gravitational field of the host has no effect on the RAR. This is in contrast with the external field effect in modified Newtonian dynamics (MOND) which causes galaxies in strong external fields to deviate from the RAR. This difference between ΛCDM and MOND offers a possible way to discriminate between them.

Scaling-up fermentation of Escherichia coli for production of recombinant P64k protein from Neisseria meningitidis

Background: P64k is a Neisseria meningitidis high molecular weight protein present in meningococcal vaccine preparations. The lpdA gene, which encodes for this protein, was cloned in Escherichia coli and the P64k recombinant protein was expressed in E. coli K12 GC366 cells under the control of a tryptophan promoter. P64k was expressed as an intracellular soluble protein about 28% of the total cellular protein. Several scale-up criteria of fermentation processes were studied to obtain the recombinant P64k protein at the pilot production scale. Results: The best operational conditions at a larger scale production of P64k recombinant protein were studied and compared using the four following criteria: Constant Reynold's number (Re constant), Constant impeller tip speed (n di constant), Constant power consumption per unit liquid volume (P/V constant) and Constant volumetric oxygen transfer coefficients (KLa/k constant). The highest production of the recombinant protein was achieved based on the constant KLa/k scale-up fermentation criterion, calculating the aeration rate (Q) and the impeller agitation speed (n) by iterative process, keeping constant the KLa/k value from bench scale. The P64k protein total production at the 50 l culture scale was 546 mg l -1 in comparison with the 284 mg l -1 obtained at 1.5 l bench scale. Conclusions: The methodology described herein, for the KLa/k scale-up fermentation criterion, allowed us to obtain the P64k protein at 50 l scale. A fermentation process for the production of P64k protein from N. meningitidis was established, a protein to be used in future vaccine formulations in humans.

Escala de felicidad para adultos (EFPA) / Happines scale for adults (EFPA)

Estudiar la felicidad psicométricamente es reciente, y los escasos instrumentos disponibles son de raíz inglesa. Felicidad es un concepto no consensuado aún, típicamente medido como unidimensional, mediante pocos ítems directos, y generalmente sin especificarse qué lo constituye. En el Estudio 1 y basados en un concepto pentadimensional y emic de felicidad, fue construida una escala de 100 ítem para medirla en adultos chilenos. Aplicada a diferentes muestras (n=68; n=277) y mediante análisis factorial exploratorio fue depurada, dando origen a la Escala de Felicidad Para Adultos (EFPA) con 21 ítem de buena confiabilidad y validez, distribuidos en cuatro dimensiones: estado psicológico, tener familia, orientación de logro y optimismo. En un Estudio 2 fue realizada una validación cruzada de la EFPA en una nueva muestra de adultos (n=341), y mediante análisis paralelo y ecuaciones estructurales se probó diferentes modelos, confirmándose uno de cuatro y otro de tres dimensiones, optándose por éste último: estado, tener familia y orientación de logro

Studying happiness psychometrically is recent, and the few available instruments are English rooted. Happiness is a concept that hasn't reached to an agreement yet, typically measured as unidimensional, through a few direct items, and usually not specifying what constitutes it. In study 1, and based on a pentadimensional and emic concept of happiness, a 100 items scale was built to measure it among Chilean adults. It was applied to different samples (n=68; n=277) and refined through exploratory factor analysis, giving origin to the Happiness Scale for Adults (EFPA) -composed by 21 items- with good reliability and validity, distributed among four dimensions: psychological state, having family, achievement orientation and optimism. In study 2, the EFPA crossed validity was carried out with a new sample of adults (n=341), and through parallel analysis and structural equation modelling various models were tested, being confirmed one of 4 and other of 3 dimensions, keeping the later: state, having family and achievement orientation.

Variable transcriptional responsiveness of the P2X3 receptor gene during CFA-induced inflammatory hyperalgesia.

The purinergic receptor P2X3 (P2X3-R) plays important roles in molecular pathways of pain, and reduction of its activity or expression effectively reduces chronic inflammatory and neuropathic pain sensation. Inflammation, nerve injury, and cancer-induced pain can increase P2X3-R mRNA and/or protein levels in dorsal root ganglia (DRG). However, P2X3-R expression is unaltered or even reduced in other pain studies. The reasons for these discrepancies are unknown and might depend on the applied traumatic intervention or on intrinsic factors such as age, gender, genetic background, and/or epigenetics. In this study, we sought to get insights into the molecular mechanisms responsible for inflammatory hyperalgesia by determining P2X3-R expression in DRG neurons of juvenile male rats that received a Complete Freund's Adjuvant (CFA) bilateral paw injection. We demonstrate that all CFA-treated rats showed inflammatory hyperalgesia, however, only a fraction (14-20%) displayed increased P2X3-R mRNA levels, reproducible across both sides. Immunostaining assays did not reveal significant increases in the percentage of P2X3-positive neurons, indicating that increased P2X3-R at DRG somas is not critical for inducing inflammatory hyperalgesia in CFA-treated rats. Chromatin immunoprecipitation (ChIP) assays showed a correlated (R2 = 0.671) enrichment of the transcription factor Runx1 and the epigenetic active mark histone H3 acetylation (H3Ac) at the P2X3-R gene promoter in a fraction of the CFA-treated rats. These results suggest that animal-specific increases in P2X3-R mRNA levels are likely associated with the genetic/epigenetic context of the P2X3-R locus that controls P2X3-R gene transcription by recruiting Runx1 and epigenetic co-regulators that mediate histone acetylation.